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. 2022 Aug 30;13:999470. doi: 10.3389/fimmu.2022.999470

Figure 6.

Figure 6

Dyslipidemia was aggravated in IL-10MUT/MUT hamsters on HFD. (A) Body weight curve of WT and IL-10MUT/MUT hamsters on HFD for 12 weeks (n=5/group). (B) Body temperature of WT and IL-10MUT/MUT hamsters on HFD (n=5/group). (C, D and E) Plasma TC (C), TG (D) and HDL-C (E) from WT and IL-10MUT/MUT hamsters on HFD (n=5/group). (F) Representative Western blots of plasma ApoB, ApoE and ApoA1 from WT and IL-10MUT/MUT hamsters on HFD. (G, H) Pooled plasma from the two groups were analyzed by FPLC. Triglyceride (E) and cholesterol (F) contents in different fractions of pooled plasma from WT and IL-10MUT/MUT hamsters on HFD (n = 6/group). (I) Representative Western blots of ApoB (top), ApoE (middle) and ApoA1 (bottom) in different fractions. (J, K) Plasma MDA (J) and 8-isoprostane (K) concentration from WT and IL-10MUT/MUT hamsters on HFD (n=5/group). (L) Oil red O stainings of liver tissue of WT and IL-10MUT/MUT hamsters on HFD. Bars: 50 μm. Arrows indicate lipid accumulation. (M) Hepatic TC and TG contents were measured in HFD-fed animals. (n=6~7/group). (N) Plasma LPS levels from WT and IL-10MUT/MUT hamsters on HFD (n=5/group). All values are means ± SEM, **, p<0.01. (O) Immunohistochemistry of CD68 in the liver from WT (left) and IL-10MUT/MUT (right) hamsters on HFD. Bars: 50 μm; arrows indicate positive stainings. (P) Cryo-sections of liver from WT (top) and IL-10MUT/MUT (bottom) hamsters on HFD were double-stained with CD68 (green) and LPS (red) antibodies. Nuclei were stained with DAPI (blue). Bars: 100 μm; white arrows indicate positive stainings. (Q) TUNEL staining (green) in the iWAT from WT (top) and IL-10MUT/MUT (bottom) hamsters on HFD. Nuclei were stained with DAPI (blue). Bars: 100 μm; white arrows indicate positive stainings. (R) Expression levels of inflammatory factors in liver were determined by real-time PCR (n=5/group). All values are means ± SEM, **p<0.01. All date were expressed as means ± SEM, *p<0.05; **p<0.01.