TABLE 2.
Growth of the W. succinogenes mutants by anaerobic respiration with formate as the electron donor at 37°C
| Strain | Doubling timea (h) for growth with:
|
||||
|---|---|---|---|---|---|
| Fumarate | Fumarateb | Malateb | Aspartateb | Nitrate + succinate | |
| Wild type | 1.3 | 1.0 | 1.1 | 1.2 | 1.4 |
| DcuA− DcuB− mutant | ∞ | ∞ | ∞ | ∞ | 1.5 |
| DcuA− mutant | 2.0 | 1.3 | 1.4 | 1.4 | 1.5 |
| DcuB− mutant | 1.9 | 1.0 | 1.0 | 1.3 | 1.5 |
| DctQM− mutant | 1.7 | 1.1 | 2.2 | ||
Average of at least five measurements; the standard deviation did not exceed 10%.
The complex medium also contained brain heart infusion (1.3%, wt/vol) (Gibco BRL).