TABLE 2.
Disproportionation kinetic parameters of wild-type CGTase and Y217F
| Enzyme |
Kcat (min−1) |
KmA (hesperidin) (g L−1) |
KmB (maltodextrin) (g L−1) |
Kcat/KmA (L g−1 min−1) |
|---|---|---|---|---|
| Wild type | 0.47 | 6.52 | 0.92 | 0.07 |
| Y217F | 4.85 | 10.86 | 2.11 | 0.45 |
Disproportionation kinetic parameters of wild-type CGTase and Y217F
| Enzyme |
Kcat (min−1) |
KmA (hesperidin) (g L−1) |
KmB (maltodextrin) (g L−1) |
Kcat/KmA (L g−1 min−1) |
|---|---|---|---|---|
| Wild type | 0.47 | 6.52 | 0.92 | 0.07 |
| Y217F | 4.85 | 10.86 | 2.11 | 0.45 |