TABLE 3.
Primers used for point mutations
| Primersa | Sequence (5′−3′ direction) xxx: mutation site |
Target mutation (codon) | |||||
|---|---|---|---|---|---|---|---|
| F205-R | tatgaggatagcatttaccgcaatctgtat | L | V | Y | H | ||
| F205-F | gtaaatgctatcctcatagctgctxxxatccgt | (taa) | (aac) | (ata) | (atg) | ||
| Y217-R | gcggattacgatctgaataacaccgtgatg | S | R | H | F | ||
| Y217-F | attcagatcgtaatccgccagatcxxxcagatt | (gct) | (gcg) | (atg) | (aaa) | ||
| A252-R | cacatgagcgaaggctggcagactagcctg | L | V | F | G | T | S |
| A252-F | ccagccttcgctcatgtgtttaacxxxatccac | (cag) | (cac) | (aaa) | (gcc) | (ggt) | (gct) |
| K254-R | ttgatggcattcgcgtggatgcggttxxxcacatgagcgaag | H | R | L | S | I | |
| K254-F | cacgcgaatgccatcaatgcctttgtccagcca | (cat) | (cgc) | (ctg) | (agc) | (att) | |
| H255-R | gaaggctggcagactagcctgatgagcgatatt | Y | S | R | F | ||
| H255-F | gctagtctgccagccttcgctcatxxxtttaac | (gta) | (gct) | (gcg) | (gaa) | ||
| F281-R | agcggcgaagttgatccgcagaaccatcat | L | V | H | Y | ||
| F281-F | cggatcaacttcgccgctgcccagxxxccattc | (taa) | (cac) | (atg) | (ata) | ||
| G283-R | ggcgaagttgatccgcagaaccatcattttgcg | S | V | T | L | F | |
| G283-F | gttctgcggatcaacttcgccgctxxxcagaaa | (gct) | (cac) | (ggt) | (cag) | (aaa) | |
a
F: forward, R: reverse.