TABLE 2.
Different doses of MecVax protein antigens CFA/I/II/IV MEFA protein (strain 9472) and toxoid fusion 3xSTaN12S-mnLTR192G/L211A protein (strain 9471) used in mouse s.c. or i.m. immunization studiesa
| Mouse immunization route and MecVax antigen | MecVax dose (μg) |
||||
|---|---|---|---|---|---|
| Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | |
| s.c. (n = 6) | |||||
| CFA/I/II/IV MEFA | 10 | 25 | 20 | 25 | 25 |
| 3xSTaN12S-mnLTR192G/L211A | 25 | 10 | 25 | 20 | 25 |
| i.m. (n = 10) | |||||
| CFA/I/II/IV MEFA | 12 | 6 | 3 | 1 | |
| 3xSTaN12S-mnLTR192G/L211A | 12 | 6 | 3 | 1 | |
a
dmLT adjuvant (0.2 μg) was included in the immunized groups. s.c., subcutaneous; i.m., intramuscular. Control animals received no MecVax.