TABLE 4.
Kinetic values of AoCel5A, AiCel5A, and mutants with CMC-Na as the substrate
| Enzyme | sp act (U/mg) | Km (mg/ml) | Vmax (μmol/min·mg) | Kcat (s–1) | Kcat/Km (ml/s/mg) |
|---|---|---|---|---|---|
| AiCel5A | 1,157 ± 17 | 5.74 ± 0.4 | 2,228 ± 77 | 1,188 ± 43 | 207 ± 21 |
| T85D | 1,379 ± 37 | 5.67 ± 0.3 | 2,413 ± 67 | 1,407 ± 38 | 248 ± 19 |
| Q236A | 1,281 ± 28 | 6.17 ± 0.7 | 2,328 ± 57 | 1,358 ± 32 | 220 ± 23 |
| D308P | 1,520 ± 16 | 4.98 ± 0.3 | 2,664 ± 87 | 1,420 ± 43 | 285 ± 29 |
| AoCel5A | 1,069 ± 21 | 7.94 ± 0.5 | 1,403 ± 61 | 818 ± 31 | 117 ± 19 |
| T105D | 905 ± 23 | 7.34 ± 0.9 | 1,333 ± 57 | 777 ± 30 | 105 ± 17 |
| N214A | 1,091 ± 27 | 7.51 ± 0.6 | 1,593 ± 64 | 870 ± 34 | 123 ± 18 |
| Q256A | 968 ± 21 | 7.37 ± 0.6 | 1,370 ± 77 | 799 ± 39 | 108 ± 19 |
| V321P | 1,112 ± 27 | 6.90 ± 0.5 | 1,522 ± 76 | 888 ± 39 | 128 ± 39 |
| D328P | 1,755 ± 18 | 6.74 ± 0.4 | 2,056 ± 83 | 1,199 ± 45 | 178 ± 31 |
| V321P/D328P | 1,839 ± 22 | 6.68 ± 0.4 | 2,173 ± 81 | 1,267 ± 49 | 189 ± 27 |
| N214A/V321P/D328P | 1,881 ± 31 | 6.93 ± 0.3 | 2,210 ± 71 | 1,289 ± 52 | 186 ± 31 |