TABLE 2.

F. columnare growth in TYES broth with iron chelator^a

Strain	Amt of chelator (deferiprone) needed to prevent growth, μMb,c	Amt of iron (FeCl3) needed to restore turbid growthc,d
Wild type	728	100
ΔiucA	345	40
ΔiucC	345	40
ΔiucX	586	60
ΔiucA ΔiucC	96	10
ΔiucX ΔiucA ΔiucC	75	10
ΔsidL	50	10
ΔsidL ΔiucX	50	10

^aTYES, tryptone yeast extract salts.

^bThe amounts listed indicate the minimum concentration of deferiprone that prevented F. columnare growth.

^cTubes were incubated in a rotator for 24 h at 28°C.

^dThe minimum concentration of ferric chloride added to restore turbid F. columnare growth in the presence of chelator.