TABLE 4.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Plasmid constructed using this primer |
|---|---|---|
| 2465 | GCTAGGGTACCACCGCAGAGTTTTGGTTGAA | pRC44 |
| 2466 | GCTAGGGATCCCTTGGTTTTTGGGTTTTTCAG | pRC44 |
| 2467 | GCTAGGGATCCAGCAAATTTGTTTGCAGTCCC | pRC45 |
| 2468 | GCTAGGTCGACTGCATCGCCGTGTGTACTAT | pRC45 |
| 2542 | GCTAGGGTACCCATACGCCCGAAGGAGAAA | pRC49 |
| 2543 | GCTAGGGATCCGGAACACTGGCTAATCCTATTCAT | pRC49 |
| 2544 | GCTAGGGATCCTGTAAGAATACGAGCAGTTGATGT | pRC50 |
| 2545 | GCTAGGTCGACCGTCAGGAATTGGGTGATG | pRC50 |
| 2546 | GCTAGGGTACCTTACTTGCTTGTTTTAGAGAGG | pRC48 |
| 2547 | GCTAGCTGCAGCTTTGATAGTTTTTACCATACAGG | pRC48 |
| 2560 | GCTAGGGATCCAACGATTTCGTTGCTTCAGG | pRC57, RC69 |
| 2561 | GCTAGGTCGACATGCGGGTAGAAGACAAAACC | pRC57 |
| 2562 | GCTAGGTCGACGATGTTTGTTCTTAAATCTTTCCA | pRC58 |
| 2563 | GCTAGGCATGCCACAGCAACCTTATCCGT | pRC58, RC69 |
| 2564 | GCTAGGGTACCATGCAAGTAAAAGCGACATCC | pRC56 |
| 2565 | GCTAGCTGCAGAGTGCCATGTATTCACCCAAA | pRC56 |
| 2583 | GCTAAGGTACCCATTCCGACAAAAGGAGGAA | pRC64 |
| 2584 | GCTAAGGATCCAACCCTATTGCTCCGTACAAAAAA | pRC64 |
| 2585 | GCTACGGATCCTTTGCCTATCATACCCCAATACTG | pRC63 |
| 2586 | GCTAAGTCGACATTCCCAAATCATGCAGACC | pRC63, RC71 |
| 2587 | GCTATGGTACCTTTACCGTACTAACCCTACTAACG | pRC62 |
| 2588 | GCTAACTGCAGGCATATTCTTTTTGAACCCACT | pRC62 |
| 2599 | GCTATGGTACCGGCCTTCATAGTCTAGATGAAG | pRC67 |
| 2600 | GCTATGGATCCTCTAACCCTATTGCTCCGTAC | pRC67 |
| 2601 | GCTAGGGATCCCATACGCCCGAAGGAGAAA | pRC71 |
a
Underlined sequences indicate added restriction enzyme sites.