FIG 2.

Gene organization of the albofungin biosynthetic gene cluster (abf BGC) in S. tumemacerans JCM5050. (A) Map of the abf BGC. (B to E) Phylogenetic analyses for the min-PKS and selected tailoring enzymes between the abf BGC and other selected BGCs. Abf32 and Abf42 are two distinguishable ACP proteins (B). Abf2 (C), Abf4 (D), and Abf50 (E) are three essential modifier enzymes required for albofungin (compound 1) maturation, with high sequence similarity to homologous enzymes encoded in other BGCs. The multisequence was aligned using ClustalW version 2.0, and the phylogenetic tree was generated by the neighbor-joining tree method without distance corrections.