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. 2022 Aug 24;88(17):e00806-22. doi: 10.1128/aem.00806-22

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Copyright © 2022 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — LC-MS analyses for albofungin (compound 1) and chloroalbofungin (compound 2). (A) LC-MS chromatographs (UV₂₈₀ absorption) of compounds 1 and 2 from the cultures of pMK01 and six BAC constructs coexpressed with pSL01+pN1 (abf2 to abf61), pSL01+pN2 (orfB to abf61), pSL01+pN3 (orfI to abf61), pSL01+pN4 (orfO to abf61), pSL01+pN5 (orfL to abf61), pSL01+pN6 (orfK to abf61), pSL02+pN5 (orfL to abf58), or pSL03+pN5 (orfL to abf56). (B) LC-MS chromatographs of compounds 1 and 2 for four BAC strains, pMK01, pMK01ΔorfA, pMK01ΔorfA::orfA, and pMK01ΔorfA::orfA_K144A. (C) MS spectra of compounds 1 and 2.