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. 2022 Aug 24;88(17):e00806-22. doi: 10.1128/aem.00806-22

TABLE 1.

Kinetic comparison of OrfA and other FDHs

Enzymes (position, reference) kcat (min−1) KM (μM) kcat/KM (μM−1min−1)
OrfA (C-7, this work) 1.44 ± 0.25 42.02 ± 0.93 0.034 ± 0.003
RebH (C-7, 76) 0.6 ± 0.1 28.7 ± 1.3 0.02 ± 0.004
PrnA (C-7, 76) 1.1 ± 0.1 20.7 ± 0.1 0.05 ± 0.005
KtzR (C-7, 76) 0.4 ± 0.1 34.1 ± 2.1 0.01 ± 0.003
SttH (C-6, 76) 1.7 ± 0.1 25.3 ± 3.2 0.07 ± 0.01
Tar14 (C-6, 76) 0.42 ± 0.05 12.0 ± 1.9 0.04 ± 0.01
PyrH (C-5, 76) 2.4 ± 0.4 15.2 ± 4.2 0.16 ± 0.05