TABLE 3.
Primers used in this study
| Primer | Sequence (5′–3′)a | Restriction site |
|---|---|---|
| P1 | GCTTCCGGCTCGTATAATGTGTGG | |
| P2 | TGCGCGCACGAAAAGCATCAGG | |
| P3 | TTTGAATTCgtgtaggctggagctgcttc | EcoRI |
| P4 | TTTGAGCTCattccggggatccgtcgacc | SacI |
| P5 | GACGGTGCGTGCCGTCCCATTTTTTGTATTTATTGATTAACTTGATCTAAgtgtaggctggagctgcttc | |
| P6 | AACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGGCTTCCGGCTCGTATAATGTGTGG | |
| P7 | TCAATGAATATGGCCGCCG | |
| P8 | ATCGGCGTCGGTCATTCC | |
| P9 | GCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGgtgtaggctggagctgcttc | |
| P10 | ATAAGCGAAGCGCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTattccggggatccgtcgacc | |
| P11 | TTATGCCAGCCTGGCAAC | |
| P12 | ATTTTGCCGAACCACAAATG | |
| P13 | CAATACGGCAATATGGTAATTCTTCGACATCACACGGTTTCCTTGTTGTTgtgtaggctggagctgcttc | |
| P14 | TTTTATGCCCGAAGCGAGGCGCTCTAAAAGAGACAAGGACCCAAACCATGattccggggatccgtcgacc | |
| P15 | TGGGTCATCACTTCCGGAC | |
| P16 | TAAGCCAGCACGCAGTC | |
| P17 | CATCACCACAGCCAGGATCCATGCGAATTGGCATACCAAG | |
| P18 | CGCGCCGAGCTCGAATTCTTACAGAGCTTTCAGGATTGCATCC | |
| P19 | GAATTCGAGCTCGGCGCG | |
| P20 | GGATCCTGGCTGTGGTGATG | |
| P21 | GCGACTCCTGCATTAGGAAATGTGTAGGCTGGAGCTGCTTC | |
| P22 | TCCCCTATAGTGAGTCGTATTAATTCCGGGGATCCGTCGACCT | |
| P23 | ATTTCCTAATGCAGGAGTCGC | |
| P24 | TAATACGACTCACTATAGGGGACCTGTAGAAAT | |
| P25 | CTGAAAGCTCTGTAATAATTAACCTAGGCTGCTGCCACCGC | |
| P26 | TTACAGAGCTTTCAGGATTGCATCCACGC | |
| P27 | CCTATAGTGAGTCGTATTAattccggggatccgtcgacc | |
| P28 | TAATACGACTCACTATAGGGGA | |
| P29 | CCATTAGTGAGTCGTATTAattccggggatccgtcgacc | |
| P30 | TAATACGACTCACTAATGGGGA | |
| P31 | TTTATAGTGAGTCGTATTAattccggggatccgtcgacc | |
| P32 | TAATACGACTCACTATAAAGGA | |
| P33 | TATTTTAACGGAGTAACATTTAGCTCGTACATGAGCAGCTTGTGTGGCTCgtgtaggctggagctgcttc | |
| P34 | TTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATGCTGCTGCCCATGGTATATCTCCTTATT | |
| P35 | GCAACACGGGTTTCATTGG | |
| P36 | CAGCATCATTACTACTGAAGCAACT | |
| P37 | TCAGTAACGGTATGTCTACACTCGGCGTTGCGCTT | |
| P38 | CAACGCCGAGTGTAGACATACCGTTACTGATCATTAATGTTAACGGCTCG | |
| P39 | CATGGTTGCATTCTCTCAATTGAAAAAATATAACCGTACATCTGCGGTCG | |
| P40 | ATATTTTTTCAATTGAGAGAATGCAACCATGTCATATGTGCTG | |
| P41 | CATGGTTGCATTCCAGTCTTTGAAAAAATATAACCGTACATCTGCGGTCG | |
| P42 | ATATTTTTTCAAAGACTGGAATGCAACCATGTCATATGTGCTG | |
| P43 | AACCAAAGGAGGTTTAAATGTTAACAAAAGCAACAAAAGAACAAAAATCCCTTGTG | |
| P44 | TTAAACCTCCTTTGGTTTTGGATACCCCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGC | |
| P45 | AAATCTACTAAGGAGGCTATTAATGTTAACAAAAGCAACAAAAGAACAAAAATCCCTTGTG | |
| P46 | TAATAGCCTCCTTAGTAGATTTCTCGAGCCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGC | |
| P47 | TAAGGAGGTTAACTATGAGCAAAGGAGAAGAACTTTTCACT | |
| P48 | GTAATTTTCGCTGTAATTTTCATCATTAGCTGCTTTGTAGAGCTCATCCATGCCATGTG | |
| P49 | CATAGTTAACCTCCTTAGGATCCCAATGC | |
| P50 | ATTACAGCGAAAATTACGCAGATGCCAGCTAATGGCCGTCGTTTTACAACGTCG | |
| P51 | AACGACGGCCATTATTTGTAGAGCTCATCCATGCCA | |
| P52 | CTACAAATAATGGCCGTCGTTTTACAACGTC | |
| P53 | TCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGGCAGCTAATGATGAAAATTACAGCGAAAATTACG | |
| P54 | CATTCATGAGATTACCAGAAAAAAGCCGGCCGTTGGGCCGGCTCTTTTACTgtgtaggctggagctgcttc | |
| P55 | GGTTGCTTCACCATCTCCAGCATC | |
| P56 | GTACCACGACCTGAGTTTTGATTTCAG |
a
Restriction sites are indicated in italics. Underlined sequences indicate the homologous sequence with the E. coli genome. Lowercase letters indicate the homologous sequence with the pKD13 plasmid.