FIG. 3.

Improved cognitive performance in dKO mice treated at P30. The cognitive-related behavioral performance was assessed at P140. (A–C) In a Barnes maze test, the ability of mice to discover and then recall the location of an escape hole was evaluated during the learning phase (A, Training, days 1–4), after a 2-day break (B, Test, day 7), and after re-positioning of the escape hole (C, Reversal days 8 and 9). The latency to successful location of the escape hole was recorded. Data were analyzed with mixed model regression with random intercept and the fixed factors of time, group, and the interaction term of group with time. To compare learning curves between groups, the β coefficients were compared. (D) Spontaneous alternation between the arms of a Y-maze was assessed over a 5-minute period. One-way ANOVA with Tukey's multiple comparisons. The data are presented as mean, and error bars represent SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). All analyses were double blind.