FIG. 5.
Liver T3 content, T3-induced gene expression, and serum TSH and TH concentrations in dKO mice treated at P30. Liver tissue was obtained for (A) measuring T3 concentrations and for qRT-PCR analysis of T3-induced gene expression, including (B) iodothyronine Dio1, (C) Me1, and (D) Ucp2. Concentrations of hormones in serum are shown, including (E) TSH, (F) T4, (G) T3, (H), and rT3. Ratios of (I) T3/rT3 and (J) T3/T4 were calculated. Data are presented as mean, and error bars represent SEM. For (A) no significant changes were found by one-way ANOVA. Student t-test was used to compare every two treatments. For (E, H, J), Mann-Whitney non-parametric test for independent samples was used. For (B–D, F, G, I), one-way ANOVA with Tukey's multiple comparisons was used. The data are presented as mean, and error bars represent SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Dio1, deiodinase 1; Me1, malic enzyme 1; rT3, reverse T3; T4, thyroxine; TH, thyroid hormone; TSH, thyroid stimulating hormone; Ucp2, uncoupling protein 2.