TABLE 1.
Allylic primer | Incorporation (pmol/mg/min) with:
|
|||
---|---|---|---|---|
M. tuberculosis
|
M. smegmatis
|
|||
Cytosol | Membranes | Cytosol | Membranes | |
None | 0 | 0 | 0 | 0 |
DMAPP | 4.0 | 0.6 | 45 | 15 |
GPP | 25.4 | 32.2 | 215 | 188 |
ω,E,E-FPP | 5.8 | 5.7 | 72 | 160 |
ω,E,E,E-GGPP | 0.4 | 1.3 | 25 | 98 |
Reaction mixtures were as described in Materials and Methods.