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. 2000 Oct;182(20):5771–5778. doi: 10.1128/jb.182.20.5771-5778.2000

TABLE 1.

Incorporation of [14C]IPP into allylic diphosphates catalyzed by cytosol or membrane fractions prepared from M. tuberculosis or M. smegmatis in the presence of various allylic diphosphate primersa

Allylic primer Incorporation (pmol/mg/min) with:
M. tuberculosis
M. smegmatis
Cytosol Membranes Cytosol Membranes
None 0 0 0 0
DMAPP 4.0 0.6 45 15
GPP 25.4 32.2 215 188
ω,E,E-FPP 5.8 5.7 72 160
ω,E,E,E-GGPP 0.4 1.3 25 98
a

Reaction mixtures were as described in Materials and Methods.