Table 1.

Survival Times of Human PrP-Expressing Transgenic Mice Inoculated With CWD Prions From Norwegian Reindeer and Moose

Inocula		Transmission
		129MM Tg35c Mice		129VV Tg152c Mice
Prion Agent	Source Code	Attack Ratea	Survival, db	Attack Ratea	Survival, db
Reindeer CWD	16-04-V142	0/19	373, 391, 457, 509, 518, 523, 630–700 (13)	0/17	498, 513, 519, 553, 567, 629–700 (12)
Moose CWD	16-60-P138	0/20	210, 258, 263, 390, 425, 428, 441, 475, 502, 524, 533, 566, 587, 634–700 (7)	0/20	253, 370, 378, 454, 539, 592, 600–700 (14)
Moose CWD	16-60-P153	0/19	409, 423, 553, 601–700 (16)	0/19	464, 482, 526, 539, 539, 589, 596, 609–700 (12)

Abbreviations: CWD, chronic wasting disease; PrP, prion protein.

^aGroups of 20 mice were inoculated intracerebrally with 30 µL of 1% (w/v) brain homogenate. Attack rate is defined as the total number of both clinically affected and subclinically infected mice as a proportion of the number of inoculated mice. Subclinical prion infection was assessed by immunohistochemical examination of brain for abnormal PrP deposition and by sodium phosphotungstic acid precipitation of 250 µL 10% (w/v) brain homogenate and analysis for protease-resistant PrP by proteinase K digestion and immunoblotting.

^bThe interval between inoculation and culling due to intercurrent illness, senescence, or termination of the experiment at 700 days postinoculation. Death dates of individual mice are shown together with the range for mice surviving beyond 600 days with the number of mice in this range shown in parentheses. Mice culled with postinoculation periods of ≤ 200 days due to intercurrent illness (all confirmed negative for subclinical prion infection) were not included in calculating attack rates.