Cell line (Human, Female) |
HeLa |
ATCC |
|
Tested negative for Mycoplasma |
Antibody |
Anti-MPHOSPH10 (MPP10) (rabbit polyclonal) |
Novus Biologicals |
NBP1-84341 |
(1:100) |
Antibody |
Anti-Fibrillarin (mouse monoclonal) |
EMD Millipore |
MABE1154 |
(1:100) |
Antibody |
Anti-POLR1A (rabbit polyclonal) |
Novus Biologicals |
NBP2-56122 |
(1:100) |
Antibody |
Anti-UBTF (rabbit polyclonal) |
Novus Biologicals |
NBP1-82545 |
(1:100) |
Antibody |
Anti-rabbit IgG Alexa 647 (goat polyclonal) |
Invitrogen |
A-27040 |
(1:1000) |
Antibody |
Anti-mouse IgG Alexa 594 (goat polyclonal) |
Invitrogen |
A-11032 |
(1:1000) |
Recombinant DNA reagent |
RPA43 WT; pcDNA3.1(+) meGFP - RPA43 |
This paper |
RP104 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK1,2,3; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234, P274-Q284, H306-H315) |
This paper |
RP105 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK3; pcDNA3.1(+) meGFP - RPA43 (ΔH306-H315) |
This paper |
RP108 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK1,2; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234, P274-Q284) |
This paper |
RP109 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK1,3; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234, H306-H315) |
This paper |
RP110 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK2,3; pcDNA3.1(+) meGFP - RPA43 (ΔP274-Q284, H306-H315) |
This paper |
RP111 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK1; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234) |
This paper |
RP112 |
Human expression plasmid |
Recombinant DNA reagent |
RPA43 ΔK2; pcDNA3.1(+) meGFP - RPA43 (ΔP274-Q284) |
This paper |
RP113 |
Human expression plasmid |
Recombinant DNA reagent |
TCOF WT; pcDNA3.1(+) meGFP - TCOF |
This paper |
RP133 |
Human expression plasmid |
Recombinant DNA reagent |
TCOF ΔK; pcDNA3.1(+) meGFP - TCOF (ΔK1390-K1406, K1438-K1468, K1476-K1483) |
This paper |
RP157 |
Human expression plasmid |
Recombinant DNA reagent |
Recombinant RPA43 C-terminus; pGEX6p1 GST-SBP-eGFP - RPA43 (E209-end) |
This paper |
RP106 |
Bacterial expression plasmid |
Recombinant DNA reagent |
Recombinant RPA43 C-terminus ΔK1,2,3; pGEX6p1 GST-SBP-eGFP - RPA43 (E209-end) (ΔK223-P234, P274-Q284, H306-H315) |
This paper |
RP107 |
Bacterial expression plasmid |
Software, algorithm |
NumPy |
NumPy |
RRID:SCR_008633
|
1.20.1 |
Software, algorithm |
BioPython |
BioPython |
RRID:SCR_007173
|
1.78 |
Software, algorithm |
Pandas |
Pandas |
RRID:SCR_018214
|
1.2.3 |
Software, algorithm |
Mahotas |
Mahotas; https://mahotas.readthedocs.io/en/latest/
|
n/a |
1.4.11 |
Software, algorithm |
SciPy |
SciPy |
RRID:SCR_008058
|
1.6.2 |
Software, algorithm |
Scanpy |
Scanpy |
RRID:SCR_018139
|
1.6.2 |
Software, algorithm |
AnnData |
AnnData |
RRID:SCR_018209
|
0.7.5 |
Software, algorithm |
NetworkX |
NetworkX |
RRID:SCR_016864
|
2.3 |
Software, algorithm |
Matplotlib |
Matplotlib |
RRID:SCR_008624
|
3.4.1 |
Software, algorithm |
Seaborn |
Seaborn |
RRID:SCR_018132
|
0.11.1 |
Software, algorithm |
Dotplot pipeline |
This paper; https://doi.org/10.5281/zenodo.6568194
|
https://doi.org/10.5281/zenodo.6568194
|
|
Software, algorithm |
SEG |
NCBI; ftp://ftp.ncbi.nlm.nih.gov/pub/seg/seg/
|
n/a |
|
Software, algorithm |
fLPS |
PMID:29132292
|
PMID:29132292
|
|