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. 2022 Sep 13;11:e77058. doi: 10.7554/eLife.77058

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Cell line (Human, Female) HeLa ATCC Tested negative for Mycoplasma
Antibody Anti-MPHOSPH10 (MPP10) (rabbit polyclonal) Novus Biologicals NBP1-84341 (1:100)
Antibody Anti-Fibrillarin (mouse monoclonal) EMD Millipore MABE1154 (1:100)
Antibody Anti-POLR1A (rabbit polyclonal) Novus Biologicals NBP2-56122 (1:100)
Antibody Anti-UBTF (rabbit polyclonal) Novus Biologicals NBP1-82545 (1:100)
Antibody Anti-rabbit IgG Alexa 647 (goat polyclonal) Invitrogen A-27040 (1:1000)
Antibody Anti-mouse IgG Alexa 594 (goat polyclonal) Invitrogen A-11032 (1:1000)
Recombinant DNA reagent RPA43 WT; pcDNA3.1(+) meGFP - RPA43 This paper RP104 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK1,2,3; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234, P274-Q284, H306-H315) This paper RP105 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK3; pcDNA3.1(+) meGFP - RPA43 (ΔH306-H315) This paper RP108 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK1,2; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234, P274-Q284) This paper RP109 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK1,3; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234, H306-H315) This paper RP110 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK2,3; pcDNA3.1(+) meGFP - RPA43 (ΔP274-Q284, H306-H315) This paper RP111 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK1; pcDNA3.1(+) meGFP - RPA43 (ΔK223-P234) This paper RP112 Human expression plasmid
Recombinant DNA reagent RPA43 ΔK2; pcDNA3.1(+) meGFP - RPA43 (ΔP274-Q284) This paper RP113 Human expression plasmid
Recombinant DNA reagent TCOF WT; pcDNA3.1(+) meGFP - TCOF This paper RP133 Human expression plasmid
Recombinant DNA reagent TCOF ΔK; pcDNA3.1(+) meGFP - TCOF (ΔK1390-K1406, K1438-K1468, K1476-K1483) This paper RP157 Human expression plasmid
Recombinant DNA reagent Recombinant RPA43 C-terminus; pGEX6p1 GST-SBP-eGFP - RPA43 (E209-end) This paper RP106 Bacterial expression plasmid
Recombinant DNA reagent Recombinant RPA43 C-terminus ΔK1,2,3; pGEX6p1 GST-SBP-eGFP - RPA43 (E209-end) (ΔK223-P234, P274-Q284, H306-H315) This paper RP107 Bacterial expression plasmid
Software, algorithm NumPy NumPy RRID:SCR_008633 1.20.1
Software, algorithm BioPython BioPython RRID:SCR_007173 1.78
Software, algorithm Pandas Pandas RRID:SCR_018214 1.2.3
Software, algorithm Mahotas Mahotas; https://mahotas.readthedocs.io/en/latest/ n/a 1.4.11
Software, algorithm SciPy SciPy RRID:SCR_008058 1.6.2
Software, algorithm Scanpy Scanpy RRID:SCR_018139 1.6.2
Software, algorithm AnnData AnnData RRID:SCR_018209 0.7.5
Software, algorithm NetworkX NetworkX RRID:SCR_016864 2.3
Software, algorithm Matplotlib Matplotlib RRID:SCR_008624 3.4.1
Software, algorithm Seaborn Seaborn RRID:SCR_018132 0.11.1
Software, algorithm Dotplot pipeline This paper; https://doi.org/10.5281/zenodo.6568194 https://doi.org/10.5281/zenodo.6568194
Software, algorithm SEG NCBI; ftp://ftp.ncbi.nlm.nih.gov/pub/seg/seg/ n/a
Software, algorithm fLPS PMID:29132292 PMID:29132292