Figure 10. Removal of exogenous amino acids except for isoleucine sensitizes parasites to 3.

Killing action of 3 or artemisinin in either normal medium containing all amino acids (blue) or amino acid (AA) free medium, containing isoleucine (red) was measured over 72 hr and determined by SYBR Green I assay. The EC₅₀ values were calculated from three biological replicates performed in triplicate and data plotted as the mean ± standard error of the mean, with the inlay bar graphs showing the mean EC₅₀ values of these replicates with statistical significance determined using an unpaired t-test.