TABLE 1.
Oligonucleotides used for PCR amplification
| Primer | Sequencea | Description |
|---|---|---|
| Xis-1 | d(CTGAGCTAGCATGTACTTGACACTTCAGGAGTGG) | Upstream primer for xis |
| Xis-2 | d(CTGAGAATTCTCATGACTTCGCCTTCTTCCCATT) | Downstream primer for xis |
| PCKR-p | d(ATCCATCGATGCTTAGGAGG) | Upstream primer for mutagenesis |
| E19A | d(AGAAGCCTTGCAACAGTTCG) | Mutagenic primer for E19A |
| R22A | d(TGAAACAGTTGCTCGATGGGTT) | Mutagenic primer for R22A |
| R23A | d(AACAGTTCGTGCATGGGTTCGGGA) | Mutagenic primer for R23A |
| R26A | d(TCGATGGGTCGCGGAATGCAGGAT) | Mutagenic primer for R26A |
| R27A | d(TGGGTTCGGGCATGCAGGATATTC) | Mutagenic primer for R27A |
| E40A | d(GATGGAAGAGCTTATCTGTTCCAC) | Mutagenic primer for E40A |
| Omnt | d(CGGCATTTTGCTATTCC) | Used in synthesizing gel-shift DNA |
| α-Omnt | d(GATCATCTAGCCATGC) | Used in synthesizing gel-shift DNA |
a
Underlined sequences are complementary to the xis gene. Bold-faced sequences represent the alanine codons.