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. 2022 Jun 17;127(6):1014–1025. doi: 10.1038/s41416-022-01878-4

Fig. 4. F1-7 induced colon cancer cells DNA damage.

Fig. 4

a GO-BP enrichment analyses on the differentially genes. b Cells were incubated with F1-7 at different concentrations as indicated for 24 h, the cell lysates were prepared for western blot analysis to determine protein expression of γH2AX and GAPDH. c, d The results of comet assay after treatment with F1-7 for 24 h, showing the presence of comet-like tail DNA (400 ×) and the density of DNA tail were calculated. e The results of EdU assay after treatment with F1-7 for 24 h, EdU-positive cell (red) immunofluorescence assay was performed, DAPI-stained nuclei blue. The positive of cells were calculated. Data are expressed as mean ± SD (n = 3).