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. 2022 Jun 17;127(6):1014–1025. doi: 10.1038/s41416-022-01878-4

Fig. 6. F1-7 showed promising efficacy to suppress colon cancer cells proliferation.

Fig. 6

a RKO were incubated with the F1-7 at different concentrations for 24 h. The expression of α-Tubulin (green) in RKO cells was determined by immunofluorescence assay. DAPI-stained nuclei blue. b Cell adhesion assay was performed to evaluate the migration impact of F1-7 on colon cancer cells. The results were representative of three replicate experiments and data are expressed as mean ± SD. c, d Transwell assay was performed to evaluate the migration impact of F1-7 on HCT-116 and RKO. The violet was dissolved by 30% acetic acid and detected absorbance at 590 nm, the percentage of inhibition was expressed using control wells as 100%. Data are expressed as mean ± SD (n = 3).