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. 2022 Sep 13;13:5371. doi: 10.1038/s41467-022-33138-7

Fig. 1. Different metabolic preferences of WT and CD37KO human B-cell lymphomas.

Fig. 1

Seahorse analysis of glycolysis (a–c) and mitochondrial respiration (d–f) in B-cell lymphoma. Cells (WT and CD37KO BJAB) were incubated in nutrient-restricted (a) or nutrient-rich (d) medium for 2 h and subjected to an acute substrate injection (1 in a, 5 in d) of glucose (10 mM), glutamine (10 mM), or palmitate (50 µM). Continuous extracellular acidification (ECAR) (a) or oxygen consumption ratio (OCR) (d) values are shown in response to 2, 6: Oligomycin A (1 µM), 3: 2-DG (20 mM), 7: FCCP (1 µM) and 4,8: Rotenone/AntimycinA (Rot/AA) (1 µM). Substrate-induced ECAR (n = 18, p = 0.0191) (b) or OCR (n = 12, p = 0.0032) (e) were calculated as the difference between baseline and acute substrate injection (1, 5). Glycolytic reserve (c) was calculated as the difference in ECAR between baseline and Oligomycin A (2) (p = 0.0107). The spare respiratory capacity (f) was calculated as the difference in OCR between baseline and FCCP (7) (p < 0.0001). Relative ATP production (counts per second, CPS) was assessed in response to Glucose (n = 3, p = 0.0270) (g), Palmitate (n = 6, WT vs KO; p = 0.0145, KO− vs KO+; p = 0.0051) (h) and Oleic acid (n = 6, p = 0.0110) (i) after 2 h (g). Two-way unpaired t-test (b, c, g) or Two-way ANOVA with Tukey’s post hoc test (e, f, h, i) were performed to check for significant differences between indicated groups, ns: not significant, *p < 0.05, **p < 0.01, ****p < 0.0001. Error bars represent mean ± SD. Experiments were repeated three times, yielding similar results. Source data are provided as a Source Data file. See also Supplementary Fig. 1.