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. 2022 Sep 13;13:5371. doi: 10.1038/s41467-022-33138-7

Fig. 7. CD37-deficient lymphoma cells and primary human lymphomas have enhanced lipid storage capacity.

Fig. 7

Human WT and CD37KO lymphoma cells (BJAB) were stained with Bodipy 493/503 that accumulates in neutral lipid regions found in lipid droplets. Quantification of LDs in untreated conditions (n = 16, p = 0.0257) (a) and palmitate supplemented (b) human WT (n = 4) and CD37KO (n = 2) lymphoma cells using flow cytometry. Experiments were repeated three times, yielding similar results. Tissues of primary CD37-negative DLBCL (c) and CD37-positive DLBCL (e) were stained with oil red O (ORO) and counterstained with haematoxylin. Quantification of ORO-positive tumour tissues from CD37-negative (n = 7) (d) and CD37-positive (n = 15) (f) DLBCLs. Same tissues were also stained with Bodipy 493/503 for 30 min and visualised using fluorescent microscopy to show the proximity of cells with LDs to large lipid deposit (1. arrow) and a zoom of CD37 positive (2.) and negative (3.) cells (g). Three large zones were selected from each total-section tile scan and analysed for the size (CD37pos: n = 27357 LD in ten individual tissue sections, CD37neg: 6827 LD in seven individual tissue sections, p = 0.0482) (h) and amount relative to nuclei (i) of the lipid droplets. One-way paired t-test (a) and two-way unpaired t-test (h, i) revealed significant differences between indicated groups, *p < 0.05, ****p < 0.0001. Blue (squares) and red (circles) zones (b) represent the 95% Confidence interval for the trend. Error bars represent mean ± SD. Source data are provided as a Source Data file. See also Supplementary Fig. 7.