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a Simplified flowchart of two HCR steps. First, probe sets containing a split of HCR initiator detect the target lncRNA (hybridisation step). Second, HCR hairpins bind to the initiator and get longer like a chain (amplification step). b Representatives of HCR images for lncRNA HOTAIR at ×10 objective. c–e Zoomed images of HCR for lncRNAs HOTAIR (c), TUG1 (d) and CDKN2B-AS1 (e) at ×100 oil immersion objective.
