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. 2022 Jun 24;127(6):1026–1033. doi: 10.1038/s41416-022-01887-3

Fig. 3. CCL23 induces expression of exhaustion markers in CD8 + T cells.

Fig. 3

a Schematic illustrating the process of isolating CD8 + T cells from PBMCs of healthy donors and treating the cells with exogenous CCL23 for qRT-PCR and flow cytometry analysis. b Expression of exhaustion markers on CD8 + T cells by qRT-PCR after treatment with CCL23. The experiment was performed once. c CTLA-4 and PD-1 expression is upregulated on CD8 + T cells after treatment with CCL23 by flow cytometry. The experiment was performed with n = 3 donors. d Expression of CCL23 in M1- and M2-polarised macrophage supernatant. The experiment was replicated once. e Neutralisation of CCL23 in M2-conditioned media partially reverses CTLA-4 expression on CD8 + T cells by flow cytometry. The experiment was performed with n = 3 donors. All error bars are mean + /− SD. Statistical analysis was carried out by an unpaired t test (*P < 0.05; ****P < 0.0001).