Table 2.

Comparison of immunohistochemistry results (H-score) between cutaneous melanoma (CM, n = 37) and benign (melanocytic) nevi (BN, n = 10): eight proteins have been investigated relating to a prognostic gene expression assay (MelaGenix®) for CM

Antibody	CM H-score *Cases without staining (%) **Only melanocytes	BN H-score *Cases without staining (%) **Only melanocytes	P value Mann–Whitney test
COL6A6	23.5 (0–68.2) 3 (8.1) 34.8 (0–110.4)	2.8 (0–25.9) 5 (50) 5 (0–48.8)	= 0.0015  = 0.0024
DCD	0 (0–52.1) 24 (64.9) 0 (0–103.5)	0 (0–13.7) 8 (80) 0 (0–15.6)	n.s.
GBP4	31.5 (9.7–78.3) 0 (0) 48.7 (18.4–128.6)	14.8 (4.8–46.6) 0 (0) 35.5 (9.7–79.8)	= 0.0080  = 0.058***
KLHL41	73.2 (19.1–162) 0 (0) 113.3 (14.8–164.7)	46.1 (18.1–90.9) 0 (100) 74.7 (36.7–155.7)	= 0.025  = 0.061***
KRT9	0 (0–81.4) 22 (59.5) 0 (0–13.9)	0 (0–52.2) 8 (80) 0 (0–70)	n.s.
PIP	60.1 (0–108.3) 1 (2.7) 80.3 (0–166.9)	57.4 (15.1–96.6) 0 (0) 69.8 (8.1–102)	n.s.
SCGB1D2	0 (0–12.8) 28 (75.7) 0 (0–21.5)	0 (0–1.3) 5 (50) 0 (0–1)	n.s.
SCGB2A2	16.7 (0–46.7) 7 (18.9) 25.9 (0–79.6)	19.5 (6.5–47.7) 0 (0) 23.8 (7.3–49.3)	n.s.

n.s. non-significant (P > 0.1)

*Immunoreactivity > 0

**Only stained melanocytes counted

***Trend for significance