Fig. 3. Deletion of IL-1R1 preferentially inhibits Jak2V617F mutant hematopoietic stem/progenitor cells.

a A scheme on competitive BM transplantation approach to assess the effects of IL-1R1 deletion on Jak2V617F mutant hematopoietic stem/progenitors is depicted. b Percentages of donor-derived (CD45.2⁺) total, myeloid cells (Gr1⁺), B cells (B220⁺), and T cells (TCRβ⁺) in peripheral blood were measured every 4 weeks after pI-pC injection (n = 5, 4, 4, 4, 4, 4 recipient mice for Jak2^VF/+ donor, n = 7, 7, 7, 6, 6, 6 mice for IL-1R1cKO; Jak2^VF/+ donor at 0, 4, 8, 12, 16 and 20 weeks, respectively). Statistical significance was determined using multiple unpaired two-tailed t-tests. c Representative flow cytometric plots on the ratio of CD45.2⁺ vs. CD45.1⁺ LSK cells in the BM of recipient mice are shown on the left, and the percentages of donor-derived (CD45.2⁺) LSK in the BM of recipient mice (n = 5, 6) are shown in bar graphs as mean ± SEM on the right. d–f Percentages of donor-derived (CD45.2⁺) LK (d), Gr1⁺ (e) and CD41⁺ (f) cells in the BM of recipient animals at 20 weeks after pI-pC injection are shown as mean ± SEM (n = 4, 6). g–j Percentages of donor-derived (CD45.2⁺) LSK (n = 4, 5) (g), LK (n = 4, 5) (h), Gr1⁺ (n = 6, 6) (i) and CD41⁺ (n = 6, 6) (j) cells in the spleens of recipient animals are shown as mean ± SEM. k Spleen weights in the recipient animals of Jak2^VF/+ and IL-1R1cKO; Jak2^VF/+ mice BM (n = 4, 6). Statistical significances in c–k were determined using two-tailed unpaired t-test. Source data are provided as a Source Data file.