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. 2022 Jun 20;127(6):1142–1152. doi: 10.1038/s41416-022-01886-4

Fig. 1. Secreted media from Vemurafenib-resistant melanoma cell lines induces a resistance phenotype in sensitive melanoma cells.

Fig. 1

a Schematic representation of the in vitro assays. b Cell viability assay or c Wound healing of M3 and Sk-Mel-28 cells after being cultivated with their own conditioned media (CM) and from the corresponding resistant CM treated with Vemurafenib (1 μM, 48 h). d Representative images of Hoechst nuclear staining of the M3 cell line (x10 microscopic field) and percentage (%) of invasive cells into the matrigel after transwell assay with CM (Vemurafenib 1 μM, 48 h). e (Left) Schematic representation of the in vivo assay. (Right) Wound-healing assay of M3 cells cultivated with 10% of mice plasma from SCID mice with xenografts of A375, M238, A375R, M238R and Sk-Mel-28R melanoma cells treated with Vemurafenib (1 μM, 48 h). Graphs show mean + SEM. n > 3 independent experiments. Statistical analysis was performed using ANOVA and Bonferroni tests (*p < 0.05; **p < 0.01; ***p < 0.001; n.s., non-significant).