TABLE 2.
Enhancement of carotenoid accumulation in E. coli by plant cDNAs individually and in combination
| Plasmid | cDNA(s) | % of controla
|
|
|---|---|---|---|
| Lycopene | Zeaxanthin | ||
| pBluescript SK− | Control | 100 ± 4 | 100 ± 8 |
| pAalytB | Adonis lytB | 152 ± 7 | 149 ± 2 |
| pAtipiTrc | Arabidopsis ipi | 205 ± 9 | 204 ± 15 |
| pTedxs | Marigold dxs | 209 ± 8 | 223 ± 10 |
| plytB/ipiTrc | lytB, ipi | 203 ± 16 | 208 ± 21 |
| pdxs/lytB | lytB, dxs | 338 ± 23 | 342 ± 32 |
| pdxs/ipiTrc | ipi, dxs | 355 ± 14 | 345 ± 29 |
| pdxs/lytB/ipiTrc | lytB, ipi, dxs | 324 ± 8 | 306 ± 8 |
a
Values are means ± standard deviations for micrograms of carotenoid per volume of cell culture as a percentage of the empty cloning vector (pBluescript SK−) control. For extracts of the lycopene control cultures, A480.5 was 0.288 ± 0.011. For the zeaxanthin control cultures, A458.5 was 0.204 ± 0.016.