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. 2022 Aug 31;16:963169. doi: 10.3389/fncel.2022.963169

Figure 1.

Figure 1

Increased expression of DNA damage response proteins, p53bp1 and γH2ax, in cervical Wobbler spinal cord. (A,B) Exemplary immunofluorescence staining of p53bp1 (green, A) and γH2ax (green, B) and DAPI (blue) in cryo-sections of cervical wild-type and Wobbler spinal cord (p40). (C) Relative fluorescence units of p53bp1 and γH2ax in cryo-sections of wild-type and Wobbler spinal cords (p40). ImageJ 1.53f51 (National Institute of Health, USA) was used to analyze the fluorescence intensity. Intensity of DAPI channel was used for normalization. Data are provided as means ± SD including individual data points. Data were tested for significance using Student’s t-test. Significant differences are indicated by **p < 0.01, n = 5. (D) Tp53bp1- and H2ax-mRNA expression level in wild-type and Wobbler mice at p40. The qPCR was performed using five samples for each genotype. For relative quantification of p53bp1-, and H2ax-mRNA expression, the 2−ΔΔCt method was conducted using the housekeeping gene GAPDH for normalization; data are provided as means ± SD including individual data points. Data were tested for significance using Student’s t-test. Significant differences are indicated by *p < 0.05, **p < 0.01, n = 5.