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. 2022 Aug 18;21(9):e13692. doi: 10.1111/acel.13692

FIGURE 3.

FIGURE 3

B cells from older human donors are able to upregulate costimulatory molecules and activation markers after stimulation. (a–e) Representative flow cytometric histograms showing the expression levels of costimulatory molecules by memory B cells from a 29 year old (grey) and 70 year old (blue) donor after 48 h stimulation with CD40L and IL‐21 or media (orange) and graphs showing the percentages of cells positive for the marker, or geometric mean of marker using the gating strategies shown, for CD80 (a), CD86 (b), HLA–DR (c), CD69 (d) and IL‐21R (e). These were gated on live CD19+ CD20+ cells. Fluorescent minus one (FMO) controls were included for the IL‐21R stain and are shown on the representative histogram. Bar height corresponds to the mean, error bars indicate standard deviation, and each symbol represents values from independent donors. Statistics were calculated using the unpaired Mann–Whitney U test. Data were representative of three independent repeat experiments.