FIGURE 4.

B cells from aged donor mice do not have defects in proliferation after immunisation. (a) Schematic diagram of adoptive transfer experiments to compare intrinsic function of B cells from young and aged mice in young recipient mice post‐immunisation. (b) Representative flow cytometric histograms showing the cell trace violet stains of donor HEL⁺ B220⁺ cells from either a young adult (14 weeks old) or aged (98 weeks old) mouse 3 days post‐transfer and immunisation. (c) Graph showing the percentage of donor HEL⁺ B cells in each division in recipient spleens 3 days post‐transfer and immunisation. (d) Representative flow cytometric plots for gating of GL7⁺ cells. Numbers adjacent to gates indicate percentage of donor HEL⁺ B220⁺ cells. (e) Percentage and number of GL7⁺ cells derived from donor cells from young or aged mice in recipient spleens 3 days post‐transfer and immunisation. Bar graphs show the results of one of two independent experiments (n = 5–6 per group/experiment). Bar height corresponds to the mean, error bars indicate standard deviation, and each symbol represents one biological replicate. Statistics were calculated using the unpaired Mann–Whitney U test. (f) Graph showing the percentage of GL7⁺ out of HEL⁺ Donor B cells in each division. p‐Value shown was generated using two‐way ANOVA with the Sidak's multiple comparisons test. Data were representative of two independent repeat experiments.