TABLE 1.
List of antibodies used for flow cytometric analysis of human cells
| Company and clone | Identifier | Dilution | ||
|---|---|---|---|---|
| Panel for human plasma cell differentiation | ||||
| 1 | APC‐e780‐coupled Live/Dead | eBioscience | 65‐0865‐14 | 1:10000 |
| 2 | APC‐e780‐coupled anti‐human CD14 | eBioscience (61D3) | 470149‐42 | 1:400 |
| 3 | APC‐e780‐coupled anti‐human CD16 | eBioscience (eBioCB16) | 47‐0168‐42 | 1:400 |
| 4 | APC‐e780‐coupled anti‐human CD3 | eBioscience (UCHT1) | 47‐0038‐42 | 1:400 |
| 5 | BB515‐coupled anti‐human CD19 | BD (HIB19) | 564456 | 1:200 |
| 6 | APC‐coupled anti‐human CD38 | eBioscience (HIT2) | 17‐0389‐42 | 1:400 |
| 7 | BV711‐coupled anti‐human CD27 | BioLegend (0323) | 302834 | 1:400 |
| 8 | PeCy7‐coupled anti‐human CD20 | BioLegend (2H7) | 302312 | 1:400 |
| 9 | BUV737‐coupled anti‐human IgD | BD (IA6‐2) | 563813 | 1:100 |
| 10 | PE‐coupled IRF4 | BioLegend (IRF4.3E4) | 646404 | 1:200 |
| Panel for costimulatory molecules expression | ||||
| 1 | APC‐e780‐coupled Live/Dead | eBioscience #65‐0865‐14 | 65‐0865‐14 | 1:10000 |
| 2 | APC‐e780‐coupled anti‐human CD14 | eBioscience (61D3) | 470149‐42 | 1:400 |
| 3 | APC‐e780‐coupled anti‐human CD16 | eBioscience (eBioCB16) | 47‐0168‐42 | 1:400 |
| 4 | APC‐e780‐coupled anti‐human CD3 | eBioscience (UCHT1) | 47‐0038‐42 | 1:400 |
| 5 | BB515‐coupled anti‐human CD19 | BD (HIB19) | 564456 | 1:200 |
| 6 | BV711‐coupled anti‐human CD27 | BioLegend (0323) | 302834 | 1:400 |
| 7 | PeCy7‐coupled anti‐human CD20 | BioLegend (2H7) | 302312 | 1:400 |
| 8 | BV510‐coupled anti‐human IgD | BD (IA6‐2) | 563813 | 1:200 |
| 9 | PerCPe710‐coupled anti‐human IL‐21R | eBioscience (2SX21R) | 46‐3601‐4 | 1:200 |
| 10 | PE‐coupled anti‐human CD80 | eBioscience (2D10.4) | 12‐0809‐42 | 1:400 |
| 11 | PECf594‐coupled anti‐human CD86 | BD (2331) | 562390 | 1:400 |
| 12 | BUV395‐coupled anti‐human HLA‐DR | BD (G46‐6) | 565972 | 1:400 |
| 13 | BV786‐coupled anti‐human CD69 | BD (FN50) | 310932 | 1:200 |
| Panel for phenotypic analysis | ||||
| 1 | APC‐e780‐coupled Live/Dead | eBioscience | 65‐0865‐14 | 1:10000 |
| 2 | APC‐e780‐coupled anti‐human CD14 | eBioscience (61D3) | 470149‐42 | 1:400 |
| 3 | APC‐e780‐coupled anti‐human CD16 | eBioscience (eBioCB16) | 47‐0168‐42 | 1:400 |
| 4 | APC‐e780‐coupled anti‐human CD3 | eBioscience (UCHT1) | 47‐0038‐42 | 1:400 |
| 5 | BB515‐coupled anti‐human CD19 | BD (HIB19) | 564456 | 1:200 |
| 6 | APC‐coupled anti‐human CD38 | eBioscience (HIT2) | 17‐0389‐42 | 1:400 |
| 7 | BV711‐coupled anti‐human CD27 | BioLegend (0323) | 302834 | 1:400 |
| 8 | PeCy7‐coupled anti‐human CD20 | BioLegend (2H7) | 302312 | 1:400 |
| 9 | BUV3955‐coupled anti‐human IgD | BD (IA6‐2) | 563813 | 1:100 |
| 10 | PerCPe710‐coupled anti‐human IL‐21R | eBioscience (2SX21R) | 46‐3601‐4 | 1:200 |
| 11 | PE‐coupled IRF4 | BioLegend (IRF4.3E4) | 646404 | 1:200 |
| 12 | BV450‐coupled CD40 | BD (5C3) | BD 561219 | 1:400 |
| Surface stain for spectral flow cytometric analysis | ||||
| 1 | BUV563‐coupled anti‐human FcRL5 | BD (509F6) | 749598 | 1:500 |
| 2 | BUV615‐coupled anti‐human CD19 | BD (HIB19) | 751273 | 1:2000 |
| 3 | BV480‐coupled anti‐human CD21 | BD (B‐Ly4) | 746613 | 1:2000 |
| 4 | SparkNIR686‐coupled anti‐human CD20 | BioLegend (2H7) | 302366 | 1:2000 |
| 5 | ViaKrome808‐coupled Live/Dead | Beckman Coulter | C36628 | 1:2000 |
| 6 | APC‐Fire750‐coupled anti‐human IgD | BioLegend (IA6‐2) | 348328 | 1:2000 |
| Intracellular stain for spectral flow cytometric analysis | ||||
| 7 | BUV395‐coupled anti‐human CD27 | BD (L128) | 563816 | 1:2000 |
| 8 | BUV737‐coupled anti‐human CD10 | BD (HI10a) | 741825 | 1:1000 |
| 9 | AF532‐coupled anti‐human IgM | NOVUS (IM373) | NBP2‐34650AF532 | 1:1000 |
| 10 | SparkBlue574‐coupled anti‐human CD3 | BioLegend (SK7) | 344851 | 1:1000 |