FIGURE 3.

RAPA‐treatment reduced extent of inflammation. Each mouse was scored (0–4) for number of tissues (pancreas, liver, kidney, and spleen) with histologic evidence of inflammation. For analysis, numbers of mice with 3 or 4 affected tissues were combined. (a) Treatment significantly affected the extent of inflammation (p = 0.0006, ordinal logistic fit, likelihood ratio test). MET‐treated mice had the greatest extent of inflammation. RAPA‐ and RAPA/MET‐treatments significantly reduced the extent of inflammation compared to MET‐treatment (post hoc pairwise analysis, ordinal logistic fit). (b) c‐reactive protein is an indication of the intensity of systemic inflammation. Plasma c‐reactive protein was reduced in MET‐ and RAPA/MET‐treated mice. (c) RAPA‐ and RAPA/MET‐treatments reduced Itgam and Itgax expression in EPI fat and Itgax expression in ING fat, indicative of diminished myeloid cell infiltration possibly mediated in part by reduced Hmox1 expression. Lbp and Serpine1 expression was reduced by both RAPA‐ and RAPA/MET‐treatments in EPI fat, and Serpine1 expression was also reduced by both treatments in ING fat. Mean ± SE. N = 9–10 mice per treatment except N = 4 per treatment for ING fat gene expression. Significance annotations and treatment abbreviations as in Figure 1