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. 2022 Aug 31;13:962330. doi: 10.3389/fphys.2022.962330

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Copyright © 2022 Fu, Wang, Schroyen, Chen, Zhang, Wu, Li and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of rearing systems on bone histomorphometry of aged laying hens (95 weeks of age) [humerus, (A,B,E–H); femur, (C,D,I-L)]. Humeri and femurs were sectioned and stained with Goldner’s trichrome [(A,C), the CCS group; (B,D), the AVS group; red arrow, cortical bone; black arrow, trabecular bone]. (E,I) Trabecular bone volume/tissue volume (BV/TV), (F,J) Trabecular thickness (Tb.Th), (G,K) Trabecular number (Tb.N), (H,L) Trabecular separation (Tb.Sp). BV/TV, Tb.Th, Tb.N and Tb.Sp were counted under blinded analysis (n = 6). CCS, conventional caging system; AVS, aviary system.