Table 2.
Mutation of FATB, KASI and decrease in the very long chain fatty acid by CRISPR/Cas9.
| Gene name | Technique |
Promoter
of Cas9 |
Method | Phenotype |
Fatty acid
WT (%) |
Fatty acid
Mutant (%) |
Mutation type | References |
|---|---|---|---|---|---|---|---|---|
|
GmFATB1a
GmFATB1b |
CRISPR/Cas9 | 2x 35S | Agrobacterium-mediated transformation |
fatb1a, fatb1b (No difference) fatb1a:1b (Growth defects, male sterility) |
SFA (%) (32.28%) |
SFA (%) fatb1a-1 (18.66%) fatb1a-2 (21.72%) fatb1b-1 (16.87%) fatb1b-2 (16.56%) fatb1a:1b (Male sterility) |
fatb1a-1 (−1 bp) fatb1a-2 (−1 bp) fatb1b-1 (−1 bp) fatb1b-2 (−2 bp) fatb1a:1b (−1 bp, −30 bp) |
Ma et al., 2021 |
| AtKASI | CRISPR/Cas9 | Ubiquitin | Floral dipping | Smaller and shorter seedlings and semi-dwarf plants | – | – | -54 bp | Xie et al., 2019 |
| GmKASI | CRISPR/Cas9 | Ubiquitin (soybean) | Whole plant transformation | Homozygous knockout—wrinkled and shriveled seed, increase in sucrose, and decrease in oil content. | cv. Bert 18:2 (49.42–52.56%) 18:3 (7.51–8.55%) |
Homozygous mutant 18:2 (30.57–44.56%) 18:3 (15.48–16.99%) |
Edit (site1, 2) WPT677-3–35 (+10/+107,WT/WT) WPT677-3–43 (WT/−1,WT/−1) WPT677-3–44 (−1/+1,WT/+1) WPT677-3–48 (WT/−6,WT/+1) |
Virdi et al., 2020 |
| AhFatB10a | CRISPR/Cas9 | Not mentioned |
Agrobacterium-mediated transformation |
No differences | Huayu23 16:0 (13.3%) |
16:0 PT1-3 (12.13%) PT1-12 (12.25%) PT1-21 (11.31%) PT2-4 (12.11%) PT2-17 (11.99%) |
PT1-3 (1 substitution) PT1-12 (1 substitution) PT1-21 (−2 bp) PT2-4 (1 substitution) PT2-17 (1 substitution) |
Tang et al., 2022 |
| CsFAE1 | CRISPR/Cas9 | EC1.1 | Floral dipping | No differences compared to Suneson | cv. Suneson20:1 (14.4%) | Less than 1% of 20:1 | 3–3–1 (−5, −1, and −5 bp) 3–3–3 (−2, −14, and −1 bp) 3–3–4 (−2/+2, −2, and −1 bp) 3–3–14 (−2, −13, and −2 bp) |
Ozseyhan et al., 2018 |
| BnaFAE1 | CRISPR/Cas9 | Not metioned | Agrobacterium-mediated hypocotyl transformation | Decrease in the seed oil content No differences in agronomic traits |
22:1 WH3411 (34.9%) WH3417 (31.0%) GY284 (34.6%) |
22:1 WH3411 c03 (19.3%) a08c03 (0.07%) WH3417 c03 (18.8%) a08c03 (0.03%) GY284 a08c03 (0.02%) |
WH3411 c03 (−1 bp) a08c03 (−7/−7 bp) WH3417 c03 (−2, +1 bp) a08c03 (−3 bp, 1 substitution/ −2 bp) GY284 a08c03 (−12 and −2 bp) |
Liu et al., 2022 |
| TaFAE1 | CRISPR/Cas9 | Ubiquitin4-2 (Petroselinum crispum) | Floral dipping (requires vacuum infiltration) | - | 20:1 (15.0%) 22:1 (35.3%) |
20:1, 22:1 fae1-3 (0.9, 0.2%) fae1-4 (0.9, 0%) fae1-5 (1.2, 0.1%) |
fae1-3 (−4 bp) fae1-4 (+1 bp) fae1-5 (+1 bp) |
Mcginn et al., 2019 |