Table 4.
TAG lipase and increasing of TAG in vegetative tissues.
| Gene name | Technique | Method | Promoter | Phenotype in transgenic |
Oil content
(WT) |
Oil content (Transgenic) | References |
|---|---|---|---|---|---|---|---|
|
BnaSDP1 (GN078283) |
RNAi |
Agrobacterium-mediated transformation |
USP (from Vicia faba) |
No difference in FA composition Little adverse impact on seed vigour |
cv. Kumily 42.36 ± 0.12% |
43.84 ± 0.10 ~ 45.86 ± 0.13% | Kelly et al., 2013 |
| JcSDP1 | RNAi | Electroporation | JcSDP1 | No difference in seed size | - | Increased the total lipid content in endosperm (% w/w) | Kim et al., 2014 |
|
GmSDP1-1
GmSDP1-2 GmSDP1-3 GmSDP1-4 |
RNAi |
Agrobacterium-mediated transformation |
Soybean 11S globulin |
Rupture of seed coat Increased the seed weight (g/seed) Increased the 18:1 but no difference in 16:0, 18:0 and 18:3 |
Kariyutaka | Increased the seed yield (g/plant), oil yield (g/plant) | Kanai et al., 2019 |
|
GmSDP1-1
GmSDP1-2 |
RNAi |
Agrobacterium-mediated transformation |
Soybean glycinin | Seed weight of mutants are ranging from 208 to 226 mg/seed (WT-183 mg/seed) Increased the total lipid (mg/seed) Germination rate is slower than WT |
Williams82 (23.3%) |
Fatty acid content (24.3%) |
Aznar-Moreno et al., 2022 |
|
GmSDP1-3
GmSDP1-4 |
Fatty acid content (24.4%) |
||||||
| PfrSDP1 | RNAi |
Agrobacterium-mediated transformation |
2S albumin | Seed weight of mutants are ranging from 0.74 to 0.77 mg (WT-0.66 mg) |
Lipid content (228 μg per mg seed) 20:1-OH, 20:2-OH (122.2 and 7 μg per mg seed) |
Lipid content (261–271 μg per mg seed) 20:1-OH, 20:2-OH (144.8–155 and 8.9–10.8 μg per mg seed) |
Azeez et al., 2022 |
| AtDGAT1 | Overexpression | Leaf-disc Agrobacterium-mediated technique | rbcS | Decrease the amount of 18:3 and increase the amount of 18:1 | Total FA content (2.8%) |
Total FA content (~5.6%) |
Andrianov et al., 2010 |
| AtLEC2 | Alc | FA content (2.9% of dry weight) |
FA content (6.8% of dry weight) |
||||
| AtDGAT1 | Overexpression | Leaf-disc Agrobacterium-mediated technique | PtdCesA8A | Minor difference about number of branches and stem diameter Increased the amount of 18:1, 18:2, and 18:3 in stems |
- | Increased the oil bodies in pith, xylem, and cortex tissues Increased the total FA and TAG content |
Nookaraju et al., 2014 |
| AtLEC2 | |||||||
|
AtDGAT1
AtWRI1 SiOLEOSIN |
Overexpression |
Agrobacterium-mediated transformation |
CaMV 35S, RuBisCO small subunit |
No negative phenotype of development Increased the amount of 18:1, 18:2 and decreased the amount of 18:3 in leaves |
TAG content (% DW) (~0.2%) |
TAG content (% DW) (~15.8%) |
Vanhercke et al., 2014 |
| NtSDP1 | RNAi |
Agrobacterium-mediated transformation in transgenic lines (Vanhercke et al., 2014) |
enTCUP2 | Reduction of starch content | TAG content (% DW) (0.1%) |
TAG content (% DW) (~29.8%) |
Vanhercke et al., 2017 |
| AtLEC2 | Overexpression | SAG12 | TAG content (% DW) (~33.3%) |
||||
| VgDGAT1a | Overexpression | Transient expression | CaMV 35S | No difference of plant morphology but change the tuber morphology, germination rate and leaf chlorophyll content Increased the 18:2 and decreased the 18:3 |
- | TAG content (% DW) (~9.2%) |
Gao et al., 2018 |
| NtAn1 | CRISPR/Cas9 | Leaf-disc Agrobacterium-mediated technique | 2x 35S | Yellow seed coat and white flower Decreased the PAs content and stearic acid No difference of seed size, seed weight and seed number per fruit |
Lipid content (38.77 μg/seed) |
Lipid content (44.97-45.91 μg/seed) |
Tian et al., 2020b |
| AtACC1 | Overexpression | Leaf-disc Agrobacterium-mediated technique | CaMV 35S | Increased the amount of 18:2 and decreased the amount of 18:3 | Relative amount of TAG (1.2 mol %) Total FA content (1.08 mg/g FW) |
Relative amount of TAG (4.6 mol %) Total FA content (1.35–1.39 mg/g FW) |
Klaus et al., 2004 |
| AtWRI1 | Overexpression | Agrobacterium-mediated transformation |
GBSS | No difference of plant morphology but change the tuber morphology Increased the amount of 18:2 and decreased the amount of 18:3 |
- | Increase the TAG and polar lipid (nmol FA/mg DW) | Hofvander et al., 2016 |
|
AtDGAT1
AtWRI1 SiOLEOSIN |
Overexpression | Agrobacterium-mediated transformation |
CaMV 35S, patatin class I promoter B33 |
Increased the soluble sugar content and decrease the starch content Decreased the SFA and 18:3 but increase the MUFA in tuber |
TAG content (% DW) (0.03%) |
TAG content (% DW) (~3.3%) Increase the polar lipid |
Liu et al., 2017 |
|
StAGPase
StSDP1 |
RNAi | Electroporation | CaMV 35S | Incraease the total sugar content and decrease the total starch content in mature potato tuber | Total FA content in mature potato tuber (0.3%) |
Total FA content in mature potato tuber (~2.95%) |
Xu et al., 2019 |