TABLE 1.
Complete blood count (CBC) analysis. (A) Whole blood CBC analysis in C57BL/6J mice 8 weeks post‐transplantation of 360k human neural stem cell (hNSC)‐luc+/green fluorescent protein (GFP)+. CBC panel measures and data (mean ± standard deviation) for each experimental group. (B) CBC measures were compared between groups, and statistically significant comparisons are highlighted
| A. CBC results | |||||
|---|---|---|---|---|---|
| Measure | Normal range |
No IS (n = 7) |
Tac (n = 6) |
Tac mAb (n = 7) |
mAb (n = 7) |
| White blood cell count ‐ WBC (10 3 /μl) | 1.8–10.7 | 3.123 ± .5019 | 1.007 ± .5417 | 1.194 ± .7348 | 3.28 ± 1.469 |
| Neutrophil count ‐ NE (10 3 /μl) | .1–2.4 | .8957 ± .5754 | .2067 ± .1122 | .2643 ± .1295 | .6986 ± .3076 |
| Lymphocyte count ‐ LY (10 3 /μl) | .9–9.3 | 2.119 ± .4143 | .755 ± .4117 | .8714 ± .5934 | 2.471 ± 1.165 |
| Monocyte count ‐ MO (10 3 /μl) | .0–.4 | .09143 ± .02545 | .03 ± .0228 | .04429 ± .02637 | .09429 ± .07829 |
| Eosinophil count ‐ EO (10 3 /μl) | .0–.2 | .01 ± .005774 | .008333 ± .007528 | .008571 ± .006901 | .01429 ± .01272 |
| Basophil count ‐ BA (10 3 /μl) | .0–.2 | .004286 ± .005345 | .003333 ± .008165 | .004286 ± .005345 | .002857 ± .00488 |
| Red blood cell count ‐ RBC (10 6 /μl) | 6.36–9.42 | 8.579 ± .31 | 8.478 ± .8597 | 9.18 ± .7665 | 9.134 ± .4404 |
| Hemoglobin ‐ HB (g/dl) | 11.0–15.1 | 11.69 ± .3891 | 10.97 ± 1.234 | 11.91 ± .4634 | 11.73 ± .6317 |
| Hematocrit ‐ HCT (%) | 35.1–45.4 | 37.01 ± 1.471 | 35.63 ± 3.976 | 38.17 ± 1.608 | 39.47 ± 2.391 |
| Mean cell volume ‐ MCV (fL) | 45.4–60.3 | 43.14 ± .5442 | 42 ± .6663 | 41.71 ± 1.875 | 43.2 ± .8794 |
| Mean cell hemoglobin ‐ MCH (pg) | 14.1–19.3 | 13.64 ± .1902 | 12.9 ± .2098 | 13.01 ± .7151 | 12.83 ± .4751 |
| MCH concentrate ‐ MCHC (g/dl) | 30.2–34.2 | 31.59 ± .3805 | 30.78 ± .4167 | 31.21 ± 1.014 | 29.73 ± .9759 |
| Red cell distribution width ‐ RDW (%) | 12.4–27.0 | 17.74 ± .4894 | 17.18 ± .7305 | 18.24 ± 1.247 | 18.04 ± .4036 |
| Platelet count ‐PLT (10 3 /μl) | 592—2972 | 439.9 ± 197.1 | 377.3 ± 250.2 | 472.3 ± 249.2 | 662.3 ± 105.1 |
| Mean platelet volume ‐ MPV (fL) | 5.0–20.0 | 4.543 ± .4198 | 4.717 ± .6401 | 4.514 ± .7988 | 4.271 ± .1799 |
| B. CBC statistics | |||||||
|---|---|---|---|---|---|---|---|
| Group comparison | WBC | NE | LY | MO | EO | BA | RBC |
| No IS versus Tac | **** | *** | *** | * | ns | ns | ns |
| No IS versus Tac MAb | *** | *** | ** | * | ns | ns | ns |
| No IS versus MAb | ns | ns | ns | ns | ns | ns | ns |
| Tac versus Tac MAb | ns | ns | ns | ns | Ns | ns | * |
| Tac versus MAb | **** | ** | **** | ** | Ns | ns | ns |
| Tac MAb versus MAb | **** | * | **** | * | Ns | ns | ns |
*
Significant (p < .0332) by one‐way ANOVA.
**
Significant (p < .0021) by one‐way ANOVA.
***
Significant (p < .0001) by one‐way ANOVA.