FIG. 5.

Same as Fig. 4, except that the rpsA TIR has been used. Seven rpsA fragments differing in their 5′ end have been fused to lacZ, and the number below each group of four vertical bars corresponds to the 5′ boundary of the fragment used (Fig. 3). The 5′ boundary of fragment P3, which extends to nt −252, is not shown on Fig. 3. Since it carries the intact rpsAp3 (P3) promoter, it can drive β-galactosidase synthesis in the absence of IPTG. All symbols are defined as in Fig. 4.