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. 2022 Aug 31;13:905376. doi: 10.3389/fphar.2022.905376

FIGURE 4.

FIGURE 4

AIM2 knockdown regulated RA FLS migration and invasion. RA FLSs were transfected with siRNAs against AIM2 (siAIM2-1, siAIM2-3) or control siRNA (siC). (A,B) Effect of AIM2 knockdown on the migration and invasion of RA FLSs. RA FLS migration (A) and invasion (B) were measured with a Boyden chamber assay. The relative migration and invasion rates were calculated by counting migrated or invaded cells and then normalized to that in control group. Representative images (original magnification, ×100) are shown. Data show the mean ± SD of samples from at least 3 independent experiments. (C) Effect of AIM2 knockdown on lamellipodia formation of RA FLSs. White arrows indicate lamellipodia formation. (D,E) AIM2 knockdown downregulated TNF-α–induced expression of proinflammatory cytokines and MMPs. RT-qPCR was used to detect the expression of cytokines (D) and MMPs (E). Data show the mean ± SD from at least 3 independent experiments. * p < 0.05, ** p < 0.01 versus siC; # p < 0.05, ## p < 0.01 versus TNF-α + siC.