FIGURE 5.

Effect of Myr treatment or AIM2 knockdown on the activation of the AKT pathway in RA FLSs. Protein expression was detected using Western blot analysis. (A) RA FLSs were transfected with siRNAs for AIM2 (siAMI2-1, siAIM2-3) or control siRNA (siC) for 72 h and then stimulated with TNF-α (10 ng/ml) for 30 min (B) RA FLSs were treated with Myr (400 μM) or DMSO for 24 h, and then stimulated with TNF-α (10 ng/ml) for 30 min. Data (A,B) show the mean ± SD from at least 3 independent experiments. ^* p < 0.05 versus siC or DMSO, ^# p < 0.05, ^## p < 0.01 versus siC + TNF-α or DMSO + TNF-α.