FIG. 1.

Disruption of the tpp1⁺ gene from S. pombe. (A) Restriction map of the genomic region containing the tpp1⁺ gene. The solid bar and the arrow below indicate the tpp1⁺ ORF. (B) Construct used for the disruption of the tpp1⁺ gene. An internal NotI-BamHI fragment of the tpp1⁺ gene was replaced by the ura4⁺ cassette. The restriction sites X, N, and B correspond to XhoI, NotI, and BamHI sites, respectively. (C) Southern hybridization analysis of XhoI-digested genomic DNA from strains JY742 (h⁺ tpp1⁺; lane 1) and P698 (h⁹⁰ tpp1⁺; lane 3) and transformants MMP-3 (h⁺ tpp1::ura4⁺; lane 2) and MMP-5 (h⁹⁰ Δtpp1::ura4⁺; lane 4), with the complete tpp1⁺ ORF as a digoxigenin-labeled probe.