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Tpp1pHA protein levels following thermal or osmotic stress. The strain MMPI-3 was grown in YES medium to mid-log phase and harvested before or after heat (40°C) or osmotic (0.75 M NaCl) stress for the indicated times. The SDS-PAGE gels were transferred to nitrocellulose filters and incubated with anti-HA or anti-α-tubulin (loading control) antibodies.
