Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 31;13:955175. doi: 10.3389/fimmu.2022.955175

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Xiao, Liang, Witwer, Zhang, Wang and Yin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

SIRT1 regulates NF-κB activation induced by SEN EVs. (A) Workflow of the experimental design. Cells were treated with PBS (Control, ctrl), CON, or SEN EVs after being pretreated with PBS, miR-30b-5p antagonist, or SRT1720. (B) Immunoblot analysis of SIRT1 protein level of cells treated with CON or SEN EVs for a time course. (C) Immunofluorescence analysis of DAPI (blue) and p65 (green) in Raw 264.7 cells. Scale bars: 5 μm. (D) Quantification of immunofluorescence ratio of p65 (Nucleus/cytoplasm). RT-qPCR analysis of (E) Il-1β and (F) Il-6 mRNA levels of Raw 264.7 cells treated with PBS, CON, or SEN EVs and the indicated inhibitors. Data are from at least three independent experiments and are presented as the mean ± SD. **p<0.01; and ***p<0.001.