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. 2022 Aug 31;13:955175. doi: 10.3389/fimmu.2022.955175

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Copyright © 2022 Xiao, Liang, Witwer, Zhang, Wang and Yin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-30b-5p directly targets SIRT1 in macrophages. (A) miR-30b-5p target sequence in the 3’UTR of Sirt1. (B) Relative activity of firefly luciferase and Renilla luciferase after co-transfection with mimics and plasmids expressing wild-type or mutated target sites. (C) RT-qPCR analysis of Sirt1 mRNA level of cells treated with the miR-30b-5p antagonist. (D) RT-qPCR analysis of Sirt1 mRNA level of cells treated with miR-30b-5p mimics. (E) Immunoblot analysis of SIRT1 protein level of cells treated with miR-30b-5p mimics and antagonists. #1:mimics control group; #2: miR-30b-5p mimics group; #3: antagonists control group; #4: miR-30b-5p antagonists group. The data represent at least three independent experiments and are presented as mean ± SD. *p<0.05; and ***p<0.001.