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. 2022 Sep 13;12(9):e1048. doi: 10.1002/ctm2.1048

FIGURE 6.

FIGURE 6

Insulin resistance is accompanied by adipose tissue inflammation in HFD‐fed mice. Inflammation including macrophage recruitment into the obese adipose tissue was assessed by qRT‐PCR‐based gene expression analyses of (A) cytokines, chemokines, (B) the macrophage‐marker F4/80, CCR2, (C) serum levels of CCL2, (D) relative hepatic mRNA expression of leptin, and (E) release of IL‐6 from ex vivo‐cultured adipocytes. Data in A, B, D represent mean of n = 5 Il1r1 Hep−/– CD, n = 4 WT CD, n = 8 Il1r1 Hep−/– HFD and n = 7 WT HFD mice ± SEM, in C n = 4 Il1r1 Hep−/– CD, n = 4 WT CD, n = 6 Il1r1 Hep−/– HFD and n = 6 WT HFD mice ± SEM, and in E mean of duplicate cultures of n = 3 Il1r1 Hep−/– CD, n = 3 WT CD, n = 8 Il1r1 Hep−/– HFD and n = 4 WT HFD mice ± SEM. *p < .05 for Il1r1 Hep−/– versus WT and $ p < .05, $$ p < .01, $$$ p < .001 for CD versus HFD using two‐way method of ANOVA following the Bonferroni multiple comparison tests (A–E)