TABLE 2.
Oligonucleotide primers
| Primer | Sequencea |
|---|---|
| CYS116 | ACTCAggtACCGATTATGATACGC |
| CYS269 | GCTaCAAAagCTTGCTGTACTTGAAAAC |
| CYS314 | TTTAATTAAgCtTATAAAAAAAGTCGGG |
| CYS363 | GCTAAAAcaagCTtCAAATCTAAAAACTTATC |
| CYS228 | TGcgcTAAAgctTAAAAAATAAATTCAAAT |
| CYS326 (REV)b | CTcTCgTAAtaggAtCCGACTTTTTTTATAG |
| CYS662 (REV) | CGTTGGAtCcTCCCAATTATC |
| CYS382 (REV) | AGTCCCggAtCCGCTCTTGATAAGTTTTTAG |
| EcoCE | AACATTAGAATTCCGAGTTTAATTGTCATTGG |
| HindCE (REV) | GCAGCGTGCTTGAAGCTTGTGCAGC |
| ACCB1 | AATTTCAAGCTTAGGAGTGCGATTGCCCTGTTAAATATC |
| ACCB2 (REV) | AAGCTTCATGGATCCTACAATGCAGACAACTGTGTTTTC |
a
Sequences are shown 5′ to 3′. Lowercase letters show variation from the wild-type sequence. Restriction sites are underlined.
b
REV, primers oriented in reverse complement to the gene sequence.