TABLE 4.
Effect of leader deletions on cysH expression
| Strain | β-Galactosidase
activitya (MU)
|
|||
|---|---|---|---|---|
| − Sulfur | + Cystine | + Metb | Met + cystine | |
| MC-MET530 | 1,034 | 174 | 658 | 119 |
| MC-MET249 | 715 | 290 | 490 | 282 |
| MC-MET276Xc | 192 | 229 | 194 | 241 |
| MC328 | 1,135 | 1,290 | —d | — |
Cells were grown in sulfur-free minimal media containing cystine and methionine; the culture was then washed, split, and grown in the presence (+) or absence (−) of sulfur sources. Samples were taken 4 h after cultures were split.
Met, methionine.
In experiments performed with strain MC-MET276X, 0.2% xylose was added to the growth media.
—, not determined.