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. 2022 Aug 31;13:980526. doi: 10.3389/fimmu.2022.980526

Figure 2.

Figure 2

Lymphocyte composition in ofatumumab treated MS patients and healthy controls. Immunophenotyping was conducted in Ofa and HC using multi-color FACS. (A-D) T cell frequencies showed no difference between Ofa and HC but a tendency towards higher frequencies of T helper cells and less cytotoxic T cells in the Ofa group. (D, E) Ofa patients had significantly lower frequencies of B cells with near complete depletion (p<0.0001) compared to HC with higher proliferation of remaining B cells as shown with higher expression of Ki67 (p<0.0001). (F-O) T helper cell subpopulation analyses with specific focus on regulatory T cells (Tregs) including unsupervised clustering of the Treg populations (P). (Q-U) proliferation of cytotoxic T cell subpopulations was significantly higher in the Ofa group. Tcon SCM: stem cell like memory T conventional cells. Tcon CM: central memory T conventional cells. Treg: T regulatory. nTreg cells: naïve regulatory T cells. eTreg cells: effector regulatory T cells. nsTreg cells: non-supressive regulatory T cells. TM: transitional memory cells. Data were analyzed with a two-tailed Mann-Whitney U test. n=10 Ofa, n=9 HC. *p<0.05; **p<0.01; ***p<0.001; ****p < 0.0001, ns, not significant.