FIGURE 2.

CMC effectively mitigates host acute immune response to subcutaneous adenovirus infection. (a) Ad‐GFP with (red cycle) or without (yellow cycle) 1.3% (wt/vol, in PBS) CMC in 30 μl total volume of PBS were injected subcutaneously into the back of C57BL/6J mice as shown. Mice were sacrificed at 6, 24, and 72 h after injection, respectively. The appearance of skin at injection site was documented. Representative images are shown. (b) Histologic evaluation and immunohistochemcial staining. The retrieved skin tissues from injection sites were subjected to H&E staining and inflammatory cells were indicated by yellow boxes (×100) and arrows (×200) (i). The retrieved tissues were further subjected to IHC staining assays with antibodies for CD45, CD54, CD40L, CD3D, TNFα, and IL1β (ii). Representative positively stained cells are indicated with red arrows (×400). Representative results are shown